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March 23, 2021

General science biology mcq with answer for group-d 2021

  Chapter 1 - Animal Kingdom 

Q.1 which of the following has the largest brain in proportion to its body size 

1) Ant 

2) Elephant 

 3) Dolphin 

4) Human being 

ANS- DOLPHIN 

Q.2 Which one of the following eat an egg laying mammal ? 

1) Mongoose 

2) Platypus 

3) Whale 

4) Mole 

Ans - PLATYPUS 

Q.3 Cuttlefish belongs to phylum 

1) Echinodermata 

2) Mollusca 

3) Annelida 

4) Pisces 

Ans- Mollusca

Q. 4 which of the following features are used by Protista organism for locomotion? 

1) Maxilla 

2) Flagella 

3) Ephemera 

4) Chimera 

Ans - Flagella 

Q. 5 whittaker classified organisms into how many kingdoms ?

1) 3 

2) 5 

3) 7 

5) 9 

Ans - 5 

Q.6 Animals of which Phylum have jointed legs? 

1) Mollusca 

2) Nematoda 

3) Arthropoda 

4) protista 

Ans- Arthropoda 

Q.7 bacilli are bacteria which are 

1) Comma-shaped 

2) Rod-shaped 

3) Spiral 

4) Spherical 

Ans - Rod- shaped 

Q.8 The bodies of Penguins are .......  and their feet have webs making them good Swimmers .

1) Heavy 

2) Streamlined 

3) Oily 

4) Feathery 

Ans - streamlined 

Q.9 Maine coon, ragdoll and somali are the breeds of 

1) Dogs 

2) cats 

3) Elephant 

4) none 

Ans- Cats 


    Chapter 2 -  CELL 

Q. Ribosomes are sites for 

1) Protein synthesis 

2) Photosynthesis 

3) Fat synthesis 

4) Respiration 

Ans - protein synthesis 

Q. 2 The cell wall of a plant is composed of 

1) Cellulose 

2) Carbohydrate 

3) Lipids 

4) Lipoprotein 

Ans - cellulose 

Q. 3 In which stage of meiosis does synapsis take place ? 

1) Leptotene 

2) Pachytene 

3) Zygotene 

4) Metaphase - first 

Ans - Zygotene 

Q. 4 In plant tissues the cell walls of... are coagulated by Suberin which makes them impervious to gases and water molecules 

1) Cork 

2) Epidermis 

3) Stomata 

4) Phloem fibres 

Ans - cork 

Q.5 animals in which the cells are arranged in three embryonic layers are called 

1) Diploblastic 

2) Ectoderm 

3) Endoderm 

4) Triploblastic 

Ans - Triploblastic 

Q.6 Which one of the following cell organelle is known as suicide bags of a cell ? 

1) Plastids 

2) Lysosomes 

3) Endoplasmic reticulum 

4) Mitochondria 

Ans - Lysosomes

Q.7 Which of the following organelles shows similarity to a prokaryotic cell ? 

1) Mitochondria only 

2) Chloroplast 

3) Both chloroplast and mitochondria 

4) None of these 

Ans - ( 3 ) 

Q.8  during which stage of Mitosis the spindle fibres are formed ? 

1) Anaphase 

2) Metaphase 

3) Prophase 

4) Telophase 

Ans - metaphase

Q.9  The stage in cell division that consists of G1 phase , S phase , G2 phase is

1) Metaphase 

2) Interphase

3) Prophase 

4) Anaphase 

Ans - Interphase 

Q.10 The outermost covering of the plant cell is constituted by 

1) Cellulose 

2) Lignin 

3) Chitin 

4) Glycocalyx 

Ans - Cellulose 


Chapter 3 - plant diseases 

Q. 1 which of the following crops is related to Karnal Bunt disease ? 

1) Maize 

2) Pea 

3) Wheat 

4) Rice 

Ans - Wheat 

Q.2 Red rot is disease of the following crop 

1) Sugarcane 

2) Paddy 

3) Wheat 

4) Chick pea 

Ans - Sugar can

Q.3 which of the following is an example of Herbicide ? 

1) Sodium chlorate

2) Potassium permanganate

3) Bleaching powder

4) None of the above 

Ans - Sodium chlorate 

Q.4 What is the most responsible factor for plant diseases ? 

1) Fungus 

2) Bacterium 

3) Virus 

4) Protozoa 

Ans - Fungus 

Q.5 Consider the following : 

1.Birds 

2. Dust blowing 

3. Rain 

4. Wind blowing 

Which of the above spread plant diseases ? 

 1 and 3 Only 

3 and 4 only 

1, 2 and 4 only 

1, 2, 3 and 4 

Ans - 1, 2 ,3 and 4 

Q.6 the disease blast is related to which among the following crops ? 

Rice 

Wheat 

Sugarcane 

Chickpea 

Ans - Rice 

Q. 7 Which of the following plant does not belong to  the category pollution indicator plants ? 

1) Nicotiana tobacum 

1) Azalea 

3) Prunus 

4) Plankton algae 

Ans - Nicotiana tobacum 

Q. 8 Which of the following is / are  disease of sugarcane ? 

1) Black rot 

2) Black stripe 

3) Eye spot 

4) All of the above 

Ans - All of the above 

Q.9 Which of the following plant disease is caused by virus ? 

1) Fire blight 

2) Mosaic 

3) Granville wilt 

4) Soft rot 

Ans - Mosaic 


Chapter 4 - Plant Nutrition 

Q.1 the tissue that transport food in plants is

1) Xylem 

2) Phloem 

3) Parenchyma 

4) Collenchyma 

Ans - phloem 

Q. 2 Eutrophication of water body results from 

1) Depletion of oxygen

2) Enhanced growth of algae or algal blooms

3) High amounts of nitrogenous nutrients and orthophosphate

4) Submersing idols in the water body 

Ans - high amounts of nitrogenous nutrients and orthophosphate 

Q.3 those plants who depends on the other plants for their food is called as 

1) Parasite 

2) Autotroph 

3) Saprotroph 

4) Insectivorous 

Ans - Parasite 

Q.4 the biofertilizer used as supplementary food for the cattle specially for milking bovines is

1) Azotobacter 

2) Azospirillum 

3) Rhizobium 

4) Azolla

Ans - Azolla 

Q.5 Which of the following plant hormones inhibits  growth ? 

1) Cytokinins 

2) Gibberellins 

3) Abscisic acid 

4) Auxins

Ans Abscisic acid 

Q.6 The gas involved in the photosynthesis process is ________

1) Ozone 

2) Oxygen 

3) Hydrogen 

4) Carbon dioxide 

Ans - Carbon dioxide 

Q.7 Insectivorous plants grow on soil which is deficient in 

1) Nitrogen 

2) Carbon 

3) Sulphur 

4) Potassium 

Ans - Nitrogen 

Q.8 plants receive nutrients from

1) Atmosphere 

2) Chlorophyll 

3) Soil 

4) Light 

Ans - Soil 

Q.9 Free living anaerobic nitrogen fixing bacteria found in soil is 

1) Azotobacter 

2) Clostridium 

3) Rhizobium 

4) Vibrio

Ans - Clostridium 

Q.10 Deficiency of which of the following nutrient causes retarded growth in plants 

1) Iron 

2) Calcium 

3) Magnesium 

4) Copper 

Ans - Calcium 


 Chapter 5 - Plant Kingdom 

Q.1 the plants tolerant to the desert conditions are called

1) Polyphytes 

2) Xerophytes 

3) Halophytes 

4) Hydrophytes 

Ans - Xerophytes 

Q.2 Malus domestica is the scientific name for the 

1) Mango tree 

2) Pineapple plant 

3) Banana tree 

4) Apple tree

Ans - Apple tree 

Q.3  Which multicellular eukaryotes have cell wall and they do not perform photosynthesis ? 

1) Fungi 

2) Plantae 

3) Animalia 

4) Protista

Ans Fungi

Q.4 __________ are often referred to as the amphibians of the plant Kingdom 

1) Bryophyta 

2) Phanerogamae

3) Pteridophyta 

4) Thallophyta 

Ans - Bryophyta 

Q. 5 Amoeba and Paramecium are the members of 

1) Protista 

2) Mollusca 

3) Arthropoda 

4) Annelida 

Ans - Protista 

Q.6 Red algae are generally found in 

1) Mountains 

2) Cities 

3) Water bodies 

4) Deserts 

Ans - Water bodies 

Q.7 The plants which gives signal when the soil become dry 

1) Neem 

2) Tulsi 

3) Croton 

3) Hibiscus 

Ans - Croton 

Q.8 Which of the following is odd regarding the conversion of axillary bud into tendril 

1) Cucumber 

2) Pumpkin 

3) Watermelon 

4) Bougainvellea 

Ans - Watermelon 

Q.9 During cell division cap cells are formed in 

1) Oedigonium 

2) Ectocarpus 

3) Chara 

4) Polysiphonia 

Ans - Oedogonium 


Chapter 6 - Plant Tissues 

Q.1 Which of the following plant hormones controls fruit ripening ? 

1) Ethylene 

2) Zeatin 

3) Auxins 

4) Gibberellic acid 

Ans - Ethylene 

Q.2 ____________ tissue cells separate to form different types of permanent tissue 

1) Parenchyma 

2) Collenchyma 

3) Meristem 

4) Sclerenchyma 

Ans - Meristem 

Q.3 The capital lease of collenchyma tissue becomes thick due to the deposits of _________

1) Cellulose and pectin

2) Lignin and chitin

3) Pectin and chitin

4) Suberin and cellulose 

Q.4 which of the following tissues has dead cells ? 

1) Sclerenchyma 

2) Collenchyma 

3) Epithelial tissue 

4) Parechyma 

Ans - Sclerenchyma 

Q.5 The part which makes eggs is : 

1) Pistil 

2) Calyx 

3) Stamen 

4) Corolla

Ans - pistill 

Q.6 ___________is a monocot plant. 

1) Carrot 

2) Daisy 

3) Garlic 

4) Rose

Ans Garlic 

Q.7 Tap roots are commonly found in_________ 

1) Angiosperm 

2) Gymnosperm 

3) Monocots 

4) Dicots 

Ans - Dicots 

Q.8 Which of the following meristematic tissue is located in the leaves and internodes of the plant ?

1) Intercalary Meristem

2) Apical Meristem

3) Lateral Meristem

4) None of the above 

Ans - Intercalary meristem 

Q.9 Which of the following are the diverse components of phloem ? 

1) Phloem parenchyma and phloem fibres

2) Sieve tubes and companion cells

3) Both 1 and 2

4) None 1 and 2 

Ans - both 1 and 2 


Chapter 7 - Animal tissues 

Q.1 __________ is a condition that results from eating a diet in which nutrients are not enough or are too much such that it causes health problems 

1) Repletion 

2) Malnutrition 

3) Appetite 

4) Starvation 

Ans - Malnutrition 

Q.2 _____________is any food constituent or group of food constituents of the same general composition that aids in the support of animal life 

1) Enzyme 

2) Minerals 

3) Nutrient 

4) Protein

Ans - Nutrient 

Q.3 Forages that are specially grown for feeding to livestock are known as_______ 

1) Fodders 

2) Forages 

3) Silage 

4) Hay

Ans - Fodders 

Q.4  Which of the following is a nutrient that is essential to every living animal for survival ? 

1) Carbohydrates 

2) Protein 

3) Vitamins 

4) All of the above 

Ans (4 ) 

Q. 5 Which one of the following represents a group of autotrophs ? 

1) Algae, Spinach, Tomato, Banana 

2) Algae ,cuscuta, banana, mango 

3) Spinach, mushroom , tomato, yeast

4) Tulsi, algae, cuscuta, mushroom

Ans ( 1 ) 

Q.6 Herbivorous require a longer small intestine to digest__________ 

1) Proteins 

2) Fats 

3) Cellulose 

4) Vitamins

Ans - Cellulose 

Q.7 the small Colony aur are also called _________ 

1) Primary consumers 

2) Secondary consumers 

3) Tertiary consumers 

4) None 

Ans (2) 

Q.8 Chondrocytes are found in _______ 

1) Blood 

2) Epithelial tissue 

3) Nervous tissue 

4) Cartilage 

Ans ( 4 ) 

Q.9 The columnar cells that are specialised for secretion are called ___ 

1) Cuboidal epithelium 

2) Ciliated epithelium 

3) Columnar epithelium 

4) Glandular epithelium 

Ans (4) 

Q.10 Which one of the following organs is the main respiratory organ of whale ?

1) Integuments 

2) Gills 

3) Lungs 

4) Trachea 

Ans - Lungs  ( because whale are mammals ) 


Chapter 8- Scientific names of plants and Animals 

Q.1 Bos mutus is the scientific name for 

1) Wild yak 

2) Horse 

3) Ox 

4) Buffalo 

Ans (1) 

Q.2 What is scientific name of vitamin C' ? 

1) Ascorbic acid 

2) Calciferol 

3) Folic acid 

4) Retinol 

Ans (1) 

Q.3 Gazella bennettii is the scientific name of ________ 

1) Elephant 

2) Lion 

3) Chinkara 

4) Wild ass 

Ans (3) 

Q.4 What is the scientific name of peacock ? 

1) Molpastes 

2) Eudynamis 

3) Pavo cristatus 

4) Psittacula 

Ans (3) 

Q.5 Mangifera Indica is the scientific name of which of the following plants ? 

1) Cabbage 

2) Mango 

3) Potato 

4) Sunflower 

Ans (2) 

Q.6 paleontology is the study of_______ 

1) Bones 

2) Primates 

3) Fossils 

4) Birds 

Ans (3) 

Q.7 What is the botanical name of opium ? 

1) Emblica officinalis 

2) Papaver somniferum 

3) Rauvolfia serpentina 

4) Cinchona

Ans (2) 

Q.8 the world's most problematic aquatic weed also known as terror of Bengal is 

1) Lantana camara 

2) Eichornia crassipes 

3) Parthenium 

4) Cynodone dactylon 

Ans (2) 

Q.9 Robert hooke discovered cells in 1663. What was the specimen that he used to observe cells ? 

Phloem 

Cork 

RBC 

Platelets 

Ans (2) 


Chapter 09 - Animal diseases 

Q.1 blue tongue disease mostly affect which of the following animal ? 

1) Fish 

2) Birds 

3) Sheep 

4) Donkey

Ans - Sheep 

Q.2 Which of the following cattle can be affected with Rinderpest disease ? 

1) Sheep 

2) Goat 

3) Pig 

4) All of these 

Ans- (4) 

Q .3 A fowl pox disease mostly affects which of the following species ? 

1) Camels 

2) Birds 

3) Goat 

4) Pig 

Ans - (2) 

Q.4 Which test is used to identify the mastitis disease in cattle ? 

1) Widal test 

2) Tetrazolium test 

3) Strep test 

4) None 

Ans (3) 

Q.5 Which of the following animal disease is a viral disease ? 

1) Foot and mouth disease 

2) Mastitis 

3) Blackleg 

4) Haemorrhgic septicaemia 

Ans (1) 

Q.6 Deficiency of iron in diet causes which disease ? 

1) Rickets 

2) Scurvy 

3) Goiter 

4) Anaemia 

Ans - (4) 

Q.7 Preservation protection and improvement of stock and prevention of animal diseases veterinary training and practice comes under which list of the constitution ? 

1) State list 

2) Union list 

3) Concurrent 

4) None 

Ans - (1) 

Q.8 An animal that transmits a disease from infected person to other potential host is called ________ 

1) Secondary host 

2) Primary host 

3) Vector 

4) Parasite 

Ans (3) 

Q. 9 The transfer of causative agents of animal disease to human beings is known as ______ 

1) Infestation 

2) Circulation 

3) Zoonoses 

4) None of the above 

Ans - (3) 

Q.10 Which is not a viral disease ? 

1) Swine flu 

2) Tuberculosis 

3) Measles 

4) Chikengunea 

Ans - (2) 


Note - General science biology mcq with answer for group - d 2021. Most expected chaperwise mcq of Animal physiology 

Chapter - Nervous system 

Q.1 Which one of the following organs will not feel any pain on being pricked by a needle ? 

1) Skin 

2) Brain 

3) Heart 

4) Eye 

Ans Brain 

Q.2 Glial cells are the most abundant cells in : 

1) Lungs 

2) Kidney 

3) Gallbladder 

4) Nervous system 

Ans - (4) 

Q.3 The number of spinal nerves in human body are 

1) 12 pairs 

2) 31 pairs 

3) 31 

4) 12 

Ans (2) 

Q.4 Which of the following cranial nerves in man is both sensory and motor 

1) Optic 

2) Olfactory 

3) Trigeminal 

4) Auditory 

Ans (3) 

Q.5 In reflex action and reflex arc is formed by 

1) Brain ---> muscle ---> spinal cord 

2) Receptor ---> spinal cord ---> Muscle 

3) Muscle ---> Brain ---> spinal cord 

4)Muscle ---> Receptor ---> spinal cord 

Ans - (2) 

Q.6 Centre for heat touch cold and pressure are in 

1) Frontal lobe 

2) Occipital lobe 

3) Parietal lobe 

4) Frontal as well as occipital lobe 

Ans (3) 

Q.7 Which of the following is not correct for cerebellum ? 

1) It lies in the posterior region of the brain 

2) It controls movements speech , sight,  smell , taste , hearing , intelligence etc 

3) It is a part of mind brain 

4) It maintains equilibrium of the body 

Ans - (2) 

Q.8 _______ controls involuntary activities like coughing and sneezing 

1) Medulla 

2) Brain pons 

3) pons 

4) Cerebellum 

Ans - Medulla 

Q.9 What is the largest part of the human brain ? 

1) Mid brain 

2) Medulla oblongata 

3) Cerebrum 

4) Cerebellum 

Ans (3) 

Q.10 Damage to this area in the brain causes problems in speech production 

1) Frontal cortex 

2) Broca's area 

3) Wernicke's area 

4) Occipital cortex 

Ans (3) 


Chapter - Digestive system 

Q.1 How dose the pH change from the mouth to the anus in the digestive system ? 

1) Alkaline - acidic - alkaline 

2) Acidic - alkaline - acidic 

3) Acidic - alkaline 

4) Alkaline - acidic 

Ans - (3) 

Q.2 Name the layer in the wall of the stomach that contains nerves and blood vessels . 

1) Lamina propria 

2) Mucosa 

3) Muscularis 

4) Sub-mucosa 

Ans - (4) 

Q.3 The digestive juices present in the stomach helps to digest _______ 

1) Both carbohydrates and proteins 

2) Proteins only

3) Fats only 

4) Carbohydrates only 

Ans - (2) 

Q.4 Which of the following ststement is correct ? 

1) Assimilation of food takes place in large intestine 

2) Large intestine has finger like projection called villi 

3) Large intestine is wider and shorter than small intestine 

4) Absorption of digested food takes place in large intestine 

Ans (3) 

Q.5 Fat is completely digested in the 

1) Stomach 

2) Mouth 

3) Small intestine 

3) Large intestine 

Ans (2) 

Q.6 The circular muscle found at the junction of stomach and duodenum is 

1) Anal sphincter 

2) Cardiac sphincter

3) Meissner's plexus 

4) Pyloric Sphincter 

Ans - (4) 

Q.7 Which of the are not digested in buccal cavity ? 

1) Glucose 

2) Proteins 

3) Fatty acids 

4) All of these 

Ans (4) 

Q.8 The contraction and expansion of the walls of food pipe is called _________ movement . 

1) Peristalsis 

2) Gastric 

3) Oscillatory 

4) Diastolic 

Ans (1) 

Q.9 The stones present in the gall bladder affect the digestion of : 

1) Protein 

2) Fat 

3) Carbohydrate 

4) Nucleic acid 

Ans - (2) 


Chapter - Skeleton system 

Q.1 Which is the longest muscle in the body ? 

1) Soleus 

2) Gracilis 

3) Trapezius 

4) Sartorius 

Ans - (4) 

Q.2 ________ are a connective tissue which connect two bones to each other 

1) Tendons 

2) Muscle 

3) Cartilages 

4) Ligaments 

Ans - (4) 

Q. 3 As per the text Charak Samhita how many bones are there in a human body ?

1) 206 

2) 360 

3) 370 

4) 208 

Ans - (2) 

Q.4 How many bones are there in human spinal column ? 

1) 33 

2) 32 

3) 31 

4) 30 

Ans - (1) 

Q.5 Which of the following is moving skull bone ? 

1) Femur 

2) Mandible 

3) Atlas 

4) Tibia 

Ans (2) 

Q.6 Bone matrix is rich in : 

1) Phosphorous and potassium 

2) Calcium and phosphorous 

3) Fluoride and calcium 

4) Calcium and potassium 

Ans (2) 

Q. 7 The femur bones of the human body are also known as _______ 

1) Wrist bones 

2) Thigh bones 

3) Shoulder bones 

4) Collar bones 

Ans (2) 

Q.8 The ends of limbs are covered with what to reduce the friction in joints .

1) Ligament 

2) Cartilage 

3) Muscle 

4) Tendon 

Ans (2) 

Q. 9 The element found in teeth and bones is - 

1) Potassium and Calcium

2) Calcium and magnesium

3) Calcium and phosphorus

4) Phosphorous and sulphur 

Ans (3)

Q.10 In which part of the body are the tarsal bones found ? 

1) Ear 

2) Leg 

3) Head 

4) Hand 

Ans. - Leg 


Chapter - Respiratory System 

Q.1 What is the process of  removing air out of the respiratory system called ? 

Respiration 

Inhalation 

Exhalation 

None of the above 

Right Answer - Exhalation 

Q.2 Which cell of the human heart receives abundant oxygen rich blood from the lungs ? 

1) Left atrium 

2) Right ventricle 

3) Left ventricle 

4) Right atrium 

Ans (1) 

Q.3 The end product of aerobic respiration is : 

1) CO2 + CO 

2) CO2 + NO 

3) CO2 + O2 

4) CO2 + H2O 

ANS (4) 

Q.4 What is aerobic respiration ? 

1) Respiration without molecular oxygen

2) Respiration through skin

3) Respiration in presence of oxygen

4) Respirations of gills 

Ans (3) 

Q.5 During the _______ process , heat energy is formed 

1) Circulation 

2) Excretion 

3) Digestion 

4) Respiration 

Ans (4) 

Q. 6 Which of the following aquatic animals does not have gills ? 

1) Octopus 

2) Squid 

3) Clown fish 

4) Whale 

Ans (4) 

Q.7 Which of the following membrane covers the lungs ? 

1) Ciliated Epithelium 

2) Diaphram 

3) Pleura 

4) Sternum 

Ans (3) 

Q. 8 Main organ of respiratory system in human body is ___ ?

1) Lung 

2) Kidney 

3) Skin 

4) Heart 

Ans (1) 

Q.9 During respiration takes place in ___

1) Lungs and alveoli 

2) Trachea and larynx 

3) Alveoli and throat 

4) Throat and lungs 

Ans (1) 

















March 15, 2021

Types of 10 essential amino acids and classification

 • Definition of amino acid 

  • 10 essential amino acids

  • Properties of amino acids

  • Classification of amino acids

  

   Definition of amino acid 

Amino acids the simplest unit of proteins. The general formula of amino acid is as follows :        
     
             H
    R-------C-------COOH
              NH2
              

Amino acid consists of

A free NH2 (amino group )

• A free COOH  (carboxyl group )

All amino acids of proteins have the NH2 group attached to the carbon atom which is next to the  COOH group            ( Alpha carbon atom ). So all amino acids of proteins are Alpha amino acid. Proteins are built up from 20 different Alpha amino acids.









10 Essential amino acids 

These are amino acids which are not synthesized in the body. So they must be supplied inadequate amounts through diet  .                                            

The following are the 10 essential amino acids :-

  • Valine
  • Isoleucine
  • Phenylalanine
  • Histidine
  • Arginine
  • Lycine
  • Leucine
  • Methionine
  • Threonine
  • Tryptophan 

All these amino acids are essential for normal growth and maintenance of Nitrogen equilibrium. The nutritional value of protein depends on the amount of essential amino acids present in it. Casein of milk contains all these essential amino acids. So it is a complete protein. Gliadin of Maize lacks in tryptophan. So it is an incomplete protein .

Physical properties of amino acid 

• Amino acids are white crystalline substances.
•  They are soluble in water and insoluble in organic solvents.

• They vary in taste. They may be tasteless sweet or bitter.

• They have a high melting point 200 to 300 degree Celsius. 

• All naturally occurring amino acids are Levorotatory except glycine which is optically inactive. 

• Amino acids have a free amino group and free carboxyl group .

Chemical properties of amino acids

• Reactions due to COOH group

• They from esters with alcohols and salts with bases. 

•With ammonia they form the corresponding amide that is glutamic acid forms glutamine aspartic acid forms asparagine .

• Decarboxylation of amino acids forms the the corresponding amides that is histidine to histamine.

Reactions due to NH2 group 

• they form salt with acids.

• The free amino group can be acylated with other Carboxylic acids 

• The amino group can be substituted by alkyl o r aryl groups.

• The amino group can react with nitrous acid liberating nitrogen quantitatively.

Classification of amino acids

 Amino acids are classified in different ways. The following classification based on the properties of side chains is convenient 

Neutral amino acids :

  • Glycine
  • Alanine
  • Serine
  • Threonine
  • Valine
  • Leucine
  • Isoleucine                                                                                   

Acidic amino acids :

  • Aspartic amino acid
  • Glutamic acid                                                           

Basic amino acids :

  • Arginine
  • Lysine                                   

Sulphur containing amino acids :

  • Cysteine
  • Methionine 

Aromatic amino acids :

  • Phenyalanine
  • Tyrosine
  • Tryptophan

Heterocyclic amino acids :

  • Proline
  • Hydroxyproline
  • Histidine







March 14, 2021

What is enzymes and classifications

 What is enzyme, properties of enzyme, Location of enzyme, Energy of activation, Classification of enzymes, mechanism of action enzyme, factors influencing enzyme action, enzyme inhibition and cofactors of enzyme.

What is enzymes ?

Enzymes are are biological catalysts. They catalyse biochemical reactions in living systems. Almost all enzymes are proteins. There are some nucleic acids that behave like enzymes. These are called ribozymes. An enzyme like any protein has a primary structure that is amino acid sequence of the protein. An enzyme like any protein has the secondary and tertiary structure. When you look at tertiary structure you will notice that the backbone of the protein chain folds upon itself the chain criss-crosses itself and hence , many crevices or pockets are made. One such pocket is the 'active site'. An active site of an enzyme is a crevice Or pocket into which the substrate fits. Thus enzymes through their active site,catalyse reactions at a high rate.

Enzyme catalyst differ from inorganic catalysts in many ways what one major difference needs mention inorganic catalyst work efficiently at high temperature and high pressure. While enzyme get damaged at high temperature at above 40 degree Celsius. However enzyme isolated from organism who normally live under extremely high temperature that is hot vents and Sulphur Springs , are stable and retain their catalytic power even at high temperature up to 80 to 90 degree Celsius. Thermal stability is  such important quality of isolated from thermophilic organisms .







Properties of enzymes 

• Enzymes are required only in in small amounts .

• They quicken the reactions without being consumed o r lost in the process.

• Enzymes are proteins like all protein enzymes are colloidal in nature and precipitated bi salt solutions.

• They are in activated by heat and alteration of PH .

• enzymes have great specificity. Each enzyme catalyzes one particular reaction.

 Location of enzyme

 Enzymes maybe present inside the cell o r outside the cell. For example :
• enzymes of citric acid cycle are present in the mitochondria.

• Enzymes of glycolytic pathway are present in the cytoplasm.

• Enzymes for the transport of nutrients are present in the cell membrane.

Energy of activation

Enzymes catalyse various biochemical reactions. Normally, for any reaction to occur, the reacting molecules must come in contact. For this, the molecules must gain minimum amount of energy this is called energy of activation. Normally, the energy of activation can be lowered by increasing the temperature but in human body, the temperature is  constant. So enzymes act by lowering the energy of
activation at normal body temperature.

Classification of enzymes

Enzymes are classified according to the type of reaction catalyzed by them. The following are major classes of enzymes

• Oxidoreductases

These enzymes catalyse oxidation and reduction of their substrates.

Examples : Alcohal dehydrogenase , lactate dehydrogenase, glucose -6 -phosphate dehydrogenase .

• Transferases

These enzymes catalyse the transfer of of particular group from one substrate to another .
  • Examples
  • Transaminase
  • Transacylase
  • Transpeptidase

• Hydrolases

These enzymes catalyse hydrolysis of substrates .
 
Examples : carbohydrases, esterases, proteses 

• Lyases

They catalyse addition o r removal of groups from the the substrate mechanism other than oxidation reduction and hydrolysis .

Examples : carboxylase ,decarboxylase, aldolase and enolase

• Isomerases

They catalyse the conversion of compound into its isomer .

Examples : Isomerases, racemase and epimerase
 

• Ligases

They catalyse the linking together of two compounds coupled with breakdown of ATP o r GTP

Examples : DNA ligase, acetyl CoA ligase, succinyl CoA ligase.


 Mechanism of enzyme action 

Enzyme acts on a substrate and converts it into a product. The enzyme is regenerated at the end of the reaction. The sequence at which this occurs is as follows :

• All enzymes have reactive sites on their surface . The substrate fits on the reactive site of the enzyme. This produces a complex called enzyme substrate complex .

• This complex activates the substrate which has fit on the surface of the enzyme. As a result an activated complex is formed.

Factors influencing enzyme action 

• Temperature 

The rate of enzyme action increases with increase in temperature. This happens only e up to 40 degree Celsius beyond this temperature the enzyme gets denatured. So the rate of enzyme action comes down film the temperature reaches beyond 40 degree Celsius. The temperature at which enzyme action is maximum is called optimum temperature.

• PH

The rate of enzyme reaction increases with increases in pH. After the maximum rate is reached the activity decreases with further increase in pH. The pH at which enzyme action is maximum is called optimum PH.

• Concentration of enzyme

The rate of enzyme reaction increases with increase in enzyme concentration. This is true only when sufficient substrate molecules are available for combination with enzyme. When all the substrate molecules are saturated with the enzyme further increase in enzyme concentration has no effect.

• concentration of substrate

The rate of enzyme reaction increases with increase in concentration of the substrate. After a certain limit it becomes constant. So further increases  concentration has no effect .

  ENZYME INHIBITION

Enzyme inhibitors are substances which lower down the rate of enzyme reaction. They produce dear effect by acting on the coenzyme enzyme are prosthetic group. Also , they can act by inhibiting the combination of  the substrate with the enzyme.


















Enzyme inhibition is classified as follows :

• Competitive inhibition

The inhibitor and the substrate have structural similarity . So they both compete with each other to bind with the enzyme.
The inhibitor is successful in this competition. So an enzyme inhibitor complex is formed. This complex cannot lead to the formation of a product  (which can be formed only by enzyme-substrate complex ). 

Enzyme + Inhibitor <------------> E - In ------> No
Product

E + S <---------> ES -------------> product

Competitive inhibition is a reversible type of reaction. The inhibition can be reversed by adding and excess of the substrate. The excess substrate will displace the  inhibitor from the enzyme.

Example : The conversion of succinic acid to fumaric is catalysed by the enzyme succinic dehydrogenase .

• The reaction is inhibited by malonic acid which has a structural resemblance to succinic acid 

• Non -competitive inhibition

This type of inhibition does not involve competition between the substrate and the enzyme. The inhibitor affects the enzyme substrate complex and prevents its dissociation to release the product. 

ES + I --------> ESI ----------> No product
 
Example : enzymes with SH group are Non competitively inhibited by metal ions like 
Ag++ and Hg++.

This type of inhibition does not require structural similarity between the the inhibitor and the substrate. Also the inhibition cannot be reversed by excess concentration of the substrate .

•Allosteric inhibition

Allosteric site is a site other than the active site which is present in the enzyme. The inhibitor binds to the allosteric site and produces conformational changes in the enzyme. So the substrate cannot bind with the enzyme and a product cannot be formed .

Examples 

• ATP is an allosteric inhibitor of hexokinase.

 ADP is an allosteric inhibitor of pyruvate carboxylase.

CO-FACTORS OF ENZYMES

Enzymes are composed of one or several polypeptide chains. However there are a number of cases in which non protein constituents called co-factors are bound to the enzyme to make the enzyme catalytically active. In the instances, the protein portion of the enzymes is called the apoenzyme.

Three kinds of cofactors may be identified prosthetic groups,coenzymes and metal ions 

Prosthetic groups are organic compounds and our distinguished from other core factors in that they are tightly bound to the apoenzyme, heam is the prosthetic group and it is a part of the active site of the enzyme.
For Example, peroxidases and catalase whitch catalyse the breakdown of hydrogen peroxide to water and oxygen.

Coenzymes are also organic compounds but their Association with the apoenzyme is only transient usually occurring during the course of catalysis. Furthermore coenzymes serve as cofactors in a number of different enzyme catalysed reactions .The essential chemical components of many coenzymes are vitamins eg. Coenzyme nicotinamide adenine dinucleotide (NAD) and NADP contain the vitamin niacin.

A number of enzymes require metal ions for their activity which form coordination bonds with side chains at the active site and at the same time form one or more coordination bonds with the substrate eg. zinc is a cofactor for the proteolytic enzyme carboxypeptidase .

Catalytic activity is lost when the cofactor is removed from the enzyme which testifies that they play a crucial role in the catalytic activity of the enzyme. 





March 12, 2021

What are carbohydrates with Examples

 carbohydrates and its example , Sources , Classification , types of carbohydrates ,and functions of carbohydrates etc.

What are carbohydrates ?

Carbohydrate are primary produced by plants and form a very large group of naturally occurring organic compounds. Some common examples are Cane sugar ,glucose , starch etc. Most of them have general formula Cx(and were considered as hydrates of carbon from where the name carbohydrate was derived .

All simple sugars contain potential aldehyde or Ketone group Play . All compound which are made up of simple sugars also contain the potential aldehyde Ketone group . These groups may be in the free form o r in the combined form. These two groups are associated with reducing properties of sugars .









Classification of Carbohydrates 

• Monosaccharides

They contain only one molecule of sugar. They cannot be hydrolysed into father into simpler molecules the ratio of carbon hydrogen and oxygen in monosaccharides is 1 is to 2 is to 1. The names of the most in the monosaccharides end with ose .
Examples : Glucose, fructose, mannose etc. Depending on the number of carbon atoms present they are called as dioses, trioses, tetroses, pentoses and hexoses.

• Disaccharides

These are sugars containing two molecules of monosaccharides. The union occurs between the first carbon of monosaccharide and second or fourth carbon of second monosaccharide .If the linkage occurs between the reducing group (aldehyde or ketone group ) of both monosaccharides the reducing property is lost. The resulting compound in a non reducing sugar that is sucrose. It does not form osazones .

If the linkage involves the aldehyde or Ketone group of only one of the monosaccharides the aldehyde are Ketone group of other monosaccharide permits reduction. The resulting compound is reducing sugar eg. lactose and maltose. They can form osazones. Disaccharides have the general formula( C12 H22 O11 ).

Sucrose :

It is the commonly called as tables sugar. Also it is called as Cane sugar since it is obtained from sugarcane. It is composed of one molecule of glucose and one molecule of fructose. Linkage involves the aldehyde group of glucose and Ketone group of fructose. So these two reducing groups are not available for reduction. So sucrose is a non- reducing sugar.

Before hydrolysis Sucrose is dextrorotatory on hydrolysis sucrose gives one molecule of glucose and one molecule of fructose. Fructose is strongly laevorotatory than glucose which is the dextrorotatory . Live rotation induced by the presence of fructose is called inversion the product of hydrolysis which contains both glucose and fructose is called invertsugar .

Maltose :

It is composed of two molecules of glucose. Linkage occurs between C1 of one glucose molecule and C4 of the Other glucose molecule. Show the aldehyde group of second glucose molecule is free and available for reduction. Maltose is a reducing sugar. It can form osazone with phenylhydrazine. Germinating cereals and malt are the Natural sources of maltose. Maltose is the intermediate product in The breakdown of starch by the enzyme the amylase in the intestinal tract .
 

 Lactose :

It is present in milk. So it is commonly called as milk sugar. It contains one molecule of galactose and one molecule of glucose. The linkage occurs between C1 of galactose and C4 of glucose. So the aldehyde group of glucose is free and it is available for reduction. Lactose is a reducing sugar. So it can form osazones.

• Oliogosaccharides 

They yield 2 to 10 molecules of monosaccharides on hydrolysis. Depending on the number of monosaccharides units present they are called trisaccharide and tetrasaccharide etc. They can have reducing property if they contain free aldehyde or ketone group not involving the linkage. The reducing property decreases as the number of of carbohydrate units increases. Antibodies and coagulation factors contain oligosaccharide units .

• Polysaccharides

They yield more than 10 molecules of monosaccharides on hydrolysis . They can be considered as condensation products of several monosaccharide units joined together by glycosidic linkage. They have high molecular weight and are colloidal in nature. Polysaccharides are classified as : 

Homopolysaccharides :- if they yield one type of monosaccharide on hydrolysis that is starch, glycogen ,cellulose .

heteropolysaccharides :- if they ILD more than one type of monosaccharide on hydrolysis eg. Heparin and hyaluronic acid.

STARCH :- Starch is a homopolysaccharide containing several units of glucose. It is a storage form of carbohydrate of plants. It is found abundantly in roots ,tubers, vegetables, fruits and cereals. In these sources, starch occur in the form of granules. Starch granules contain two types of polymeric units of glucose called amylose and amylopectin .

Amylose is a straight chain polymer. It contains glucose unit linked by Alpha -1-4 glycosidic linkage. It gives blue colour with dilute iodine solution.

Amylopectin is a branched chain polymer. The glucose units are linked by Alpha 1-4 glycosidic linkage in the straight chain like amylose. But at points of branching it is alpha 1 -6 glycosidic linkage. Amylopectin gives a reddish violet colour with iodine solution .

GLYCOGEN :- it is a storage form of carbohydrate in animals so it is called as animal starch. It occurs in in animal tissues especially in liver and muscles. It is also present in yeast and fungi which do not have chlorophyll system. The molecular weight of glycogen is very high and it is about 5,000,000.
Glycogen is a polymer of glucose. In the straight chain the glycosidic linkage is  1-4 whereas at branch points it is 1-6. A branch point occurs for every 12 to 18 glucose units.

CELLULOSE :- It is a structural polysaccharide .It is found in fibrous parts of plants. It consists of long unbranched chain of Beta D - glucose molecules United by 1-4 linkage.
Cellulose is not sweet in taste. Also it does not give colour with Iodine. It is insoluble and resistant to hydrolysis. Because of its inert nature It is not digested in in the intestine. So it increases the bulk of fecal matter. The bulk increases the peristalsis and helps in defecation .







March 11, 2021

WHAT IS PROTEINS ?








 Definition of protein ,Role of protein ,Classification of protein ,protein deficiency disease 

Definition of protein 

The word protein is derived from the Greek word protios which means of first importance .Proteins are the most important constituents of all living matter they are the fundamental structural constituents of the body .

Proteins are high molecular weight polypeptides containing Alpha amino acids joined together by peptide linkage.

 Composition :

Proteins are complex organic molecules. Their weight may range from 6000 to many Millions.

They contain the elements carbon ,hydrogen oxygen, nitrogen and sometimes Phosphorus and sulphur .

Role of proteins 

      • They provide structural framework cells and tissues .

      • They act as enzymes and hormones 
  • Some transport proteins carry specific substances across membranes or body fluids .
  • Storage proteins bind with specific substances like vitamins and minerals and mediate their cellular action . 
  • Proteins can be catabolized to release energy . 
  • Proteins exert osmotic pressure which helps in maintaining electrolyte and water balance . 

      

    Classification of proteins 

Proteins are classified into the following groups :

• simple protein

• conjugated protein

• Derived protein

   Simple protein 

The simple protein simple sub-group are as follows :

  • Albumin e.g. egg and serum 
  • Globulin  
  • Glutelin  eg. Oryzenin of rice 
  • Prolamines eg. gliadin of wheat

  • Sclero proteins eg. Keratin of hairs and nails ,collagen of bone and skin 


  • Histones eg. nucleo histones and globin of haemoglobin.

  • Protamines eg. Cells of certain fish 

     Conjugated protein 

 These are  simple proteins combined with non-protein group called prosthetic group. On the basis of prosthetic group the conjugated proteins are classified as follows

• Nucleo proteins  eg. Virus proteins

 • Phosphoproteins eg. casein of milk


• Glycoproteins     eg. mucin of saliva

• Lipoproteins        eg. blood and egg yolk

• Metalloproteins   eg. ferritin which contains iron .

• Chromoproteins   eg. heamoglobin and cytochromes .

 

3. DERIVED PROTEINS 

There are two types :

1. Primary derived proteins 

 Example : metaproteins

2. Secondary  derived proteins

 Example : proteoses ,peptones ,peptides


   Sources of proteins

The two main dietary sources of protein are

 • Animal sources like milk ,meat,  egg ,cheese et. These proteins contain all the the eight essential amino acids .

• vegetable sauces like pulses , cereals, beans, nuts, cakes of oilseeds etc. They are poor in essential amino acid.

° The requirement of protein for an Indian adult it is  1 gram per kg body weight









  Functions of proteins 

• proteins are necessary for body building since they are essential constituents of of all tissues.

• They are necessary for repair and maintenance of body tissues


• They are necessary for the synthesis of antibodies hemoglobin enzymes and hormones


• The help in cell mediated immunity  and bactericidal action of leukocytes.


• Also there sources of energy (1gram yields 4 calories ) 

Structure of proteins 

The structure of proteins is very complex but well organised. The structure of proteins can be conveniently considered under the following different levels of organisation .

1.primary structure

Primary structure refers to the sequence of amino acids  which are linked through peptide bonds. The peptide Bond occurs between the carboxyl group of one amino acid and amino group .

2. Secondary structure 

Secondary structure refers to the the quilling of the polypeptide chain into a helical structure

In this structure there is hydrogen bonding between neighbouring amino acid which occurs at the sites where the peptide chain is folded to form a helix .

Three Types of secondary structures are possible :

•  Alfa-helical structure

• Reverse turn

• pleated structure


3. Tertiary Structure 

Tertiary structure refers to the folding of protein chain to form tight and compact three dimensional structure .

It is only such as confirmation which is biologically active. Protein in this confirmation is called native protein.

Such an intricate folding on the tertiary structure brings closer the the amino acids which are for away. 

This structure is further strengthened by the following forces :

• Hydrophobic interactions

• Hydrogen bonds 

• Ionic bonds

• Disulfide bonds


4. Quaternary structure 

It is the IV level of organisation in protein structure. It is shown by protein containing more than one polypeptide chain

These peptide chains are further strengthened by ionic bond hydrogen bond hydrophobic interactions etc.


Protein deficiency diseases

Various diseases are caused by dietary deficiency of proteins and amino acids some disease are caused by abnormal metabolism of proteins .

Dietary deficiency of proteins 

Deficiency of protein in diet produce two important diseases in children. They are kwashiorkar and marasmus .

• Kwashiorkar 

It is a disease of protein energy malnutrition. It occur in one year above children when they change from breast feeding to a diet low in protein .

The features are :

• Retarded growth and generalized edema

• Skin changes like pigmentation thickening cracks and ulceration

• Hair is thin and Spares the colour is radish to Grey.

Note :- diet rich in protein like milk egg and Soybean are helpful in the treatment of kwashiorkar .

      

• Marasmus 

 It occurs  below 1 year in children .It occurs due to a  diet very low in protein and calories . It occurs due to  early termination of breast feeding and feeding with very dilute Cow's milk .

The features are :

• The child is very thin with no subcutaneous fat.

• Muscles are severely wasted .

• Head is large and Limbs are thin like sticks

• Weight of the child it is greatly reduced .

• Unlike kwashiorkar there is no oedema.

 NOTE :- marasmus is is treated in the the same way as kwashiorkar by providing diet rich in protein .

Abnormal metabolism of proteins 

There are following few such diseases :

• Phenylketonuria

• Alkaptonuria 

• Albinism 

• Tyrosinosis


         Phenylketonuria

It is a metabolic disorder of protein .It occurs due to the absence of the enzyme phenylalanine hydroxylase . So phenylalanine cannot be converted to tyrosine .So this lead increased excretion of phenylalanine and it's catabolites like phenylpyruvic acid and phenyl lactic acid. The manifestation of this disease are mental retardation and seizures. Also diminished pigmentation are hair and skin can occur .

It is treated by giving a diet with very low levels phenylalanine .Tyrosine constitutes an essential amino acid in these patients and it must be provided in diet .

    Alkaptonuria

It is a metabolic disorder associated with abnormal metabolism of tyrosine. It is caused by the absence of the enzyme homogentisate oxidase. So homogentisic acid accumulates in the tissues and blood and also it appears in urine. Urine containing homogentisic turns black in colour due to oxidation and exposed to air.

Alkaptonuria does not produce in clinical manifestation. In chronic patients it may produce darkening of the cartilage and tendons. This condition is called as ochronosis .